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Article

A primer-guided nucleotide incorporation assay in the genotyping of apolipoprotein E.

Genomics - 1 Dec 1990

Syvänen A C, Aalto-Setälä K, Harju L, Kontula K, Söderlund H

Abstract excerpt

We describe a new technique by which single base changes in human genes can be conveniently detected. In this method the DNA fragment of interest is first amplified using the polymerase chain reaction with an oligonucleotide primer biotinylated at its 5'-end. The amplified 5'-biotinylated DNA is immobilized on an avidin matrix and rendered single-stranded. The variable nucleotide in the immobilized DNA is...

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PMID: 2276739
DOI: 10.1016/0888-7543(90)90255-s

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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