A primer-guided nucleotide incorporation assay in the genotyping of apolipoprotein E.

doi:10.1016/0888-7543(90)90255-s

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Article

A primer-guided nucleotide incorporation assay in the genotyping of apolipoprotein E.

Genomics - 1 Dec 1990

Syvänen A C, Aalto-Setälä K, Harju L, Kontula K, Söderlund H

Abstract excerpt

We describe a new technique by which single base changes in human genes can be conveniently detected. In this method the DNA fragment of interest is first amplified using the polymerase chain reaction with an oligonucleotide primer biotinylated at its 5'-end. The amplified 5'-biotinylated DNA is...

Topics

Apolipoproteins E
Base Sequence
Codon
DNA, Single-Stranded
Genetic Techniques
Genotype
Humans
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Polymorphism, Genetic
Templates, Genetic

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