Article
A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates.
Journal of clinical microbiology - 1 Aug 1998
Rupnik M, Avesani V, Janc M, von Eichel-Streiber C, Delmée M
Abstract excerpt
Two hundred nineteen Clostridium difficile isolates from 22 serogroups were screened for changes in the genes coding for toxin B (tcdB) and toxin A (tcdA). Parts of the toxin genes were amplified, and the PCR fragments were checked for length polymorphisms and cut with several restriction enzymes...
Topics
- Bacterial Proteins
- Bacterial Toxins
- Bacterial Typing Techniques
- Clostridioides difficile
- DNA, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Enterotoxins
- Genetic Variation
- Humans
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Serotyping
- Virulence