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Article

An efficient route to human bispecific IgG.

Nature biotechnology - 1 Jul 1998

Merchant A M, Zhu Z, Yuan J Q, Goddard A, Adams C W, Presta L G, Carter P

Abstract excerpt

Production of bispecific IgG (BsIgG) by coexpressing two different antibodies is inefficient due to unwanted pairings of the component heavy and light chains. To overcome this problem, heavy chains were remodeled for heterodimerization using engineered disulfide bonds in combination with previously identified "knobs-into-holes" mutations. One of the variants, S354C:T366W/Y349'C:T366'S:L368'A:Y407++ +'V, gave near...

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Identifiers

PMID: 9661204
DOI: 10.1038/nbt0798-677

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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