Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination.

doi:10.1016/s1097-2765(00)80101-x

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Article

Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination.

Molecular cell - 1 Jun 1998

Hiom K, Gellert M

Abstract excerpt

The 12/23 rule requires that V(D)J recombination only occurs between recombination signals with 12 and 23 base pair spacers. We show that the 12/23 rule is established prior to DNA cleavage, by the formation of a synaptic complex containing both 12-spacer and 23-spacer signals. The RAG1 and RAG2...

Topics

Base Composition
DNA
DNA-Binding Proteins
Gene Rearrangement
Genes, Immunoglobulin
High Mobility Group Proteins
Homeodomain Proteins
Immunoglobulin J-Chains
Immunoglobulin Variable Region
Mutation
Protein Binding
Recombination, Genetic

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