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Article

RAG-heptamer interaction in the synaptic complex is a crucial biochemical checkpoint for the 12/23 recombination rule.

The Journal of biological chemistry - 22 Feb 2008

Nishihara Tadashi, Nagawa Fumikiyo, Imai Takeshi, Sakano Hitoshi

Abstract excerpt

In V(D)J recombination, the RAG1 and RAG2 protein complex cleaves the recombination signal sequences (RSSs), generating a hairpin structure at the coding end. The cleavage occurs only between two RSSs with different spacer lengths of 12 and 23 bp. Here we report that in the synaptic complex, recombination-activating gene (RAG) proteins interact with the 7-mer and unstack the adjacent base in the coding region. We...

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Source

Identifiers

PMID: 18089566
DOI: 10.1074/jbc.M709890200

Genes

RAG1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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RAG-heptamer interaction in the synaptic complex is a crucial biochemical checkpoint for the 12/23 recombination rule.PMID 18089566Read ↗

Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures.Semantic proximity Read ↗

Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination.Semantic proximity Read ↗

Identification and characterization of a gain-of-function RAG-1 mutant.Semantic proximity Read ↗

Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis.Semantic proximity Read ↗

Recombination signal sequences restrict chromosomal V(D)J recombination beyond the 12/23 rule.Semantic proximity Read ↗

Initiation of V(D)J recombination in vitro obeying the 12/23 rule.Semantic proximity Read ↗

Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.Semantic proximity Read ↗

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