Article
A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice.
Genes & development - 1 Jun 1992
Skarnes W C, Auerbach B A, Joyner A L
Abstract excerpt
We have confirmed that the gene trap vector pGT4.5 creates spliced fusion transcripts with endogenous genes and prevents the synthesis of normal transcripts at the site of integration. cDNA was prepared to the lacZ fusion transcript in three ES cell lines to recover endogenous exon sequences upstream of lacZ. Each of the clones detected a unique-sized endogenous transcript, as well as the fusion transcript in the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Chimera
- Cloning, Molecular
- Gene Expression
- Genetic Vectors
- Lac Operon
- Mice
- Molecular Sequence Data