Article
Development of antibody diversity in single germinal centers: selective expansion of high-affinity variants.
European journal of immunology - 1 Oct 1994
Ziegner M, Steinhauser G, Berek C
Abstract excerpt
In a T cell-dependent immune response the microenvironment of the germinal center plays a crucial role in the affinity maturation of the antigen-specific, immunoglobulins. In order to look at the development of antibody diversity we have isolated single germinal centers and sequenced light chains...
Topics
- Amino Acid Sequence
- Animals
- Antibody Affinity
- Antibody Diversity
- B-Lymphocytes
- Base Sequence
- Clone Cells
- DNA Primers
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Genes, Immunoglobulin
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mutation
- Oxazolone
- Sequence Alignment
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid