Mutations at the domain interface of GSalpha impair receptor-mediated activation by altering receptor and guanine nucleotide binding.

Abstract excerpt

G protein alpha subunits consist of two domains, a GTPase domain and a helical domain. Receptors activate G proteins by catalyzing replacement of GDP, which is buried between these two domains, with GTP. Substitution of the homologous alphai2 residues for four alphas residues in switch III, a region that changes conformation upon GTP binding, or of one nearby helical domain residue decreases the ability of alphas...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Enzyme Activation
• Share to GTP-Binding Proteins
• Share to Models, Molecular