Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis.

Abstract excerpt

A gene coding for a putative alpha-glucosidase has been identified in the open reading frame yvdL (now termed malL), which was sequenced as part of the Bacillus subtilis genome project. The enzyme was overproduced in Escherichia coli and purified. Further analyses indicate that MalL is a specific...

Topics

• Bacillus subtilis
• Bacterial Proteins
• Cloning, Molecular
• Enzyme Induction
• Enzyme Repression
• Escherichia coli
• Fructose
• Glucose
• Maltose
• Mutagenesis, Insertional
• Open Reading Frames
• Phenotype
• Recombinant Proteins
• Sucrase-Isomaltase Complex
• alpha-Glucosidases