Article
A beta-glucosidase gene (bgl3) from Streptomyces sp. strain QM-B814. Molecular cloning, nucleotide sequence, purification and characterization of the encoded enzyme, a new member of family 1 glycosyl hydrolases.
European journal of biochemistry - 15 Jul 1994
Perez-Pons J A, Cayetano A, Rebordosa X, Lloberas J, Guasch A, Querol E
Abstract excerpt
A beta-glucosidase gene (bgl3) from Streptomyces sp. QM-B814 (American Type Culture Collection 11238) has been cloned by functional complementation of a beta-glucosidase-negative mutant of Streptomyces lividans. An open-reading frame of 1440 nucleotides encoding a polypeptide of 479 amino acids w...
Topics
- Amino Acid Sequence
- Base Sequence
- Cellobiose
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- DNA, Recombinant
- Genes, Bacterial
- Glucuronates
- Hydrogen-Ion Concentration
- Kinetics
- Mass Spectrometry