Article
Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity.
FEBS letters - 27 Feb 2004
van der Veen Bart A, Potocki-Véronèse Gabrielle, Albenne Cécile, Joucla Gilles, Monsan Pierre, Remaud-Simeon Magali
Abstract excerpt
Amylosucrase is a glucosyltransferase belonging to family 13 of glycoside hydrolases and catalyses the formation of an amylose-type polymer from sucrose. Its potential use as an industrial tool for the synthesis or the modification of polysaccharides, however, is limited by its low catalytic efficiency on sucrose alone, its low stability, and its side reactions resulting in sucrose isomer formation. Therefore,...
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