Article
Molecular cloning of a maltose transport gene from Bacillus stearothermophilus and its expression in Escherichia coli K-12.
Molecular & general genetics : MGG - 10 May 1994
Liong E C, Ferenci T
Abstract excerpt
Genes responsible for maltose utilization from Bacillus stearothermophilus ATCC7953 were cloned in the plasmid vector pBR325 and functionally expressed in Escherichia coli. The 4.2 kb Bacillus DNA insert in clone pAM1750 suppressed the growth defects on maltose caused by mutations in E. coli malt...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Cloning, Molecular
- DNA, Recombinant
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Geobacillus stearothermophilus
- Maltose
- Maltose-Binding Proteins
- Membrane Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutation
- Open Reading Frames