Article
Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae.
Nucleic acids research - 25 Sept 1991
Araki H, Hamatake R K, Morrison A, Johnson A L, Johnston L H, Sugino A
Abstract excerpt
DNA polymerase II purified from Saccharomyces cerevisiae contains polypeptides with apparent molecular masses of greater than 200, 80, 34, 30 and 29 kDa, the two largest of which (subunits A and B) are encoded by the essential genes POL2 and DPB2. By probing a lambda gt11 expression library of yeast DNA with antiserum against DNA polymerase II, we isolated a single gene, DPB3, that encodes both the 34- and 30-kDa...
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