Article
A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases.
Biochemistry - 14 Apr 1998
Veltman O R, Vriend G, Berendsen H J, Van den Burg B, Venema G, Eijsink V G
Abstract excerpt
Thermostable thermolysin-like proteases (TLPs), such as the TLP of Bacillus stearothermophilus CU-21 (TLP-ste), bind calcium in one double (Ca1,2) and two single (Ca3, Ca4) calcium binding sites. The single sites are absent in thermolabile TLPs, suggesting that they are determinants of (variation...
Topics
- Amino Acid Sequence
- Bacillus cereus
- Bacterial Proteins
- Binding Sites
- Calcium
- Calcium-Binding Proteins
- Enzyme Stability
- Geobacillus stearothermophilus
- Hot Temperature
- Metalloendopeptidases
- Models, Chemical
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Denaturation
- Protein Engineering
- Sequence Homology, Amino Acid