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Article

A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases.

Biochemistry - 14 Apr 1998

Veltman O R, Vriend G, Berendsen H J, Van den Burg B, Venema G, Eijsink V G

Abstract excerpt

Thermostable thermolysin-like proteases (TLPs), such as the TLP of Bacillus stearothermophilus CU-21 (TLP-ste), bind calcium in one double (Ca1,2) and two single (Ca3, Ca4) calcium binding sites. The single sites are absent in thermolabile TLPs, suggesting that they are determinants of (variation in) TLP stability. Mutations in the Ca3 and Ca4 sites of TLP-ste indeed reduced thermal stability, but only mutations...

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PMID: 9548763
DOI: 10.1021/bi9725879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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