Article
Limited proteolysis of beta-lactoglobulin using thermolysin. Effects of calcium on the outcome of proteolysis.
International journal of biological macromolecules - 1 Feb 1994
Dufour E, Dalgalarrondo M, Haertlé T
Abstract excerpt
Bovine beta-lactoglobulin variant B was hydrolysed with thermolysin at various concentrations of calcium ions ranging between 0 and 50 mM. The changes in calcium concentration did not influence the overall rates of beta-lactoglobulin proteolysis. However, HPLC analyses of the hydrolysates, obtained at nine different calcium concentrations, indicated that the rates of production and/or lysis of 3 of 17 identified...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium
- Cattle
- Female
- Genetic Variation
- Hydrolysis
- In Vitro Techniques
- Kinetics
- Lactoglobulins
- Molecular Sequence Data
