Crystal structures of the apo- and holomutant human lysozymes with an introduced Ca2+ binding site.

Article

Crystal structures of the apo- and holomutant human lysozymes with an introduced Ca2+ binding site.

The Journal of biological chemistry - 5 Nov 1991

Inaka K, Kuroki R, Kikuchi M, Matsushima M

Abstract excerpt

The three-dimensional structures of apo- and holomutant human lysozymes (D86/92 lysozyme), in which a calcium binding site was designed and created for enhancing molecular stability by replacing both Gln86 and Ala92 with aspartic acids, were refined at 1.8-A resolution by x-ray crystallography. T...

Topics

Apoproteins
Binding Sites
Calcium
Calcium-Binding Proteins
Crystallography
Humans
In Vitro Techniques
Muramidase
Mutation
Protein Conformation
Solvents
Structure-Activity Relationship
Water
X-Ray Diffraction

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