Article
Structural determinants of the stability of thermolysin-like proteinases.
Nature structural biology - 1 May 1995
Eijsink V G, Veltman O R, Aukema W, Vriend G, Venema G
Abstract excerpt
Thermolysin is a member of a family of homologous proteinases which differ in their resistance to thermally induced unfolding and subsequent autolytic degradation. Site-directed mutagenesis studies of the thermolysin-like proteinase (TLP) from Bacillus stearothermophilus (TLP-ste) show that its reduced resistance to thermally induced autolysis, as compared to thermolysin, is due to only some of the 44 naturally...
Topics
- Amino Acid Sequence
- Endopeptidases
- Hot Temperature
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Protein Folding
- Sequence Homology, Amino Acid
- Thermolysin