Article
An autoselection system in recombinant Kluyveromyces lactis enhances cloned gene stability and provides freedom in medium selection.
Applied microbiology and biotechnology - 1 Feb 1998
Hsieh H P, Da Silva N A
Abstract excerpt
An autoselection system for increasing plasmid stability in Kluyveromyces lactis, based on the blockage of the pyrimidine de novo and salvage pathways, was investigated. In a manner analogous to that used in Saccharomyces cerevisiae, a putative "fur 1" mutation was selected in a uraA K. lactis strain using 5-fluorouracil and 5-fluorocytosine plates. Survival of the mutant required expression of a plasmid-borne...
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