An autoselection system in recombinant Kluyveromyces lactis enhances cloned gene stability and provides freedom in medium selection.

doi:10.1007/s002530051151

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Article

An autoselection system in recombinant Kluyveromyces lactis enhances cloned gene stability and provides freedom in medium selection.

Applied microbiology and biotechnology - 1 Feb 1998

Hsieh H P, Da Silva N A

Abstract excerpt

An autoselection system for increasing plasmid stability in Kluyveromyces lactis, based on the blockage of the pyrimidine de novo and salvage pathways, was investigated. In a manner analogous to that used in Saccharomyces cerevisiae, a putative "fur 1" mutation was selected in a uraA K. lactis st...

Topics

Cloning, Molecular
Culture Media
Gene Expression
Genes, Fungal
Glycoside Hydrolases
Kluyveromyces
Mutation
Plasmids
Recombinant Proteins
Transformation, Genetic
beta-Fructofuranosidase

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