Article
Construction of stable episomes in Cryptococcus neoformans.
Current genetics - 1 Jul 1998
Varma A, Kwon-Chung K J
Abstract excerpt
We report the generation of stable plasmids constructed by inserting specific DNA sequences into previously known unstable vectors. These sequences were obtained from a DNA library recovered from a previously reported stable minichromosome created by electroporative transformation in Cryptococcus...
Topics
- Blotting, Southern
- Chromosomes, Fungal
- Cryptococcus neoformans
- DNA, Fungal
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Electroporation
- Escherichia coli
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid
