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Article

Allelic replacement in Staphylococcus aureus with inducible counter-selection.

Plasmid - 1 Jan 2006

Bae Taeok, Schneewind Olaf

Abstract excerpt

A method for rapid selection of allelic replacement mutations in the chromosome of Staphylococcus aureus is described. Plasmid pKOR1, an Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection. Plasmid transformation of staphylococci and growth at 43 degrees C, a non-permissive condition for pKOR1 replication, selects for homologous recombination and pKOR1...

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Share to Bacteriophage lambda
Share to Chromosomes, Bacterial
Share to DNA, Recombinant

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Staphylococcus aureus

Share to Tetracycline Resistance

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Share to Transformation, Genetic

Source

Identifiers

PMID: 16051359
DOI: 10.1016/j.plasmid.2005.05.005

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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