Article
New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.
Journal of bacteriology - 1 Jul 2001
Wagner R, Straub M L, Souciet J L, Potier S, de Montigny J
Abstract excerpt
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.
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