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Article

Generation of a stable non-reverting Leu- mutant of Kluyveromyces lactis by gene disruption.

Applied microbiology and biotechnology - 1 Jan 1995

Meinhardt F, Holtwick R

Abstract excerpt

The LEU2 gene coding for beta-isopropylmalate dehydrogenase of the yeast Kluyveromyces lactis strain AWJ137 was disrupted. In the resulting Leu- strain a 0.57 x 10(3)-base pairs PstI/BglII fragment of the LEU2 coding region was replaced by the TRP1 gene of Saccharomyces cerevisiae. The mutant strain was characterized by stability tests and a physical map of the disrupted region was established by...

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PMID: 7765915
DOI: 10.1007/BF00171954

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