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Article

Generation of a stable non-reverting Leu- mutant of Kluyveromyces lactis by gene disruption.

Applied microbiology and biotechnology - 1 Jan 1995

Meinhardt F, Holtwick R

Abstract excerpt

The LEU2 gene coding for beta-isopropylmalate dehydrogenase of the yeast Kluyveromyces lactis strain AWJ137 was disrupted. In the resulting Leu- strain a 0.57 x 10(3)-base pairs PstI/BglII fragment of the LEU2 coding region was replaced by the TRP1 gene of Saccharomyces cerevisiae. The mutant strain was characterized by stability tests and a physical map of the disrupted region was established by...

Topics

3-Isopropylmalate Dehydrogenase
Alcohol Oxidoreductases
Base Sequence
Biotechnology
DNA, Fungal
Genes, Fungal
Kluyveromyces
Molecular Sequence Data
Mutation
Restriction Mapping
Transformation, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 7765915
DOI: 10.1007/BF00171954

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7765915-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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