Article
Characterization of mutants of the 6'-N-acetyltransferase encoded by the multiresistance transposon Tn1331: effect of Phen171-to-Leu171 and Tyr80-to-Cys80 substitutions.
Plasmid - 1 Jan 1998
Panaite D M, Tolmasky M E
Abstract excerpt
The Klebsiella pneumoniae plasmid pJHCMW1 harbors a copy of Tn1331, a multiresistance transposon that includes the aac(6')-Ib gene which encodes a 6'-N-aminoglycoside acetyltransferase. This gene was mutagenized using the mutator Escherichia coli XL1-Red. Two plasmids with a single nucleotide mutation in aac(6')-Ib were selected for further analysis: pDP1 and pDP6. Plasmid pDP1 codes for a mutant enzyme,...
Topics
- Acetylation
- Acetyltransferases
- Amikacin
- Amino Acid Substitution
- Anti-Bacterial Agents
- DNA Transposable Elements
- Drug Resistance, Multiple
- Kanamycin
- Kanamycin Resistance
- Klebsiella pneumoniae
- Microbial Sensitivity Tests
- Mutation
- Phenylalanine
- Sequence Alignment
- Substrate Specificity
- Tyrosine