Novel Plasmid-Mediated 16S rRNA m ¹ A1408 Methyltransferase, NpmA, Found in a Clinically Isolated <i>Escherichia coli</i> Strain Resistant to Structurally Diverse Aminoglycosides

Abstract excerpt

We have isolated a multiple-aminoglycoside-resistant Escherichia coli strain, strain ARS3, and have been the first to identify a novel plasmid-mediated 16S rRNA methyltransferase, NpmA. This new enzyme shared a relatively low level of identity (30%) to the chromosomally encoded 16S rRNA methyltransferase (KamA) of Streptomyces tenjimariensis, an actinomycete aminoglycoside producer. The introduction of a...