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Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments.

Gene - 24 Nov 2004

Blaise Lydia, Wehnert Anita, Steukers Mieke P G, van den Beucken Twan, Hoogenboom Hennie R, Hufton Simon E

Abstract excerpt

Yeast display is a powerful technology for the affinity maturation of human antibody fragments. However, the technology thus far has been limited by the size of antibody libraries that can be generated, as using current transformation protocols libraries of only between 10(6) and 10(7) are typically possible. We have recently shown that Fab antibodies can be displayed on the cell surface of Saccharomyces...

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Identifiers

PMID: 15527980
DOI: 10.1016/j.gene.2004.08.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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