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Article

Fine affinity discrimination by yeast surface display and flow cytometry.

Biotechnology progress - 1 Jan 2000

VanAntwerp J J, Wittrup K D

Abstract excerpt

Yeast surface display is a eucaryotic system for the directed evolution of protein binding and stability. For antibody affinity maturation, achievable single-pass enrichment factors are a critical variable. Both reliable recovery of rare clones (yield) and effective differentiation between clones of only slightly improved affinity (purity) are paramount. To validate yeast display's purification potential, trial...

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Identifiers

PMID: 10662486
DOI: 10.1021/bp990133s

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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