Article
Why a "benign" mutation kills enzyme activity. Structure-based analysis of the A176V mutant of Saccharomyces cerevisiae L-asparaginase I.
Acta biochimica Polonica - 1 Jan 1997
Bonthron D T, Jaskólski M
Abstract excerpt
A conservative and apparently harmless A176V mutation in intracellular S. cerevisiae L-asparaginase (ScerAI) completely abolishes the enzyme activity. Sequence and structural comparisons with type II bacterial L-asparaginases show that the mutated residue is in a very conservative region and play...
Topics
- Amino Acid Sequence
- Asparaginase
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid