Article
The ASP1 gene of Saccharomyces cerevisiae, encoding the intracellular isozyme of L-asparaginase.
Gene - 24 Jun 1994
Sinclair K, Warner J P, Bonthron D T
Abstract excerpt
Saccharomyces cerevisiae produces two L-asparaginases (ASPs), intracellular ASP I and cell-wall ASP II. In this report, the ASP-I-encoding gene, ASP1, has been identified by homology cloning based on the structures of ASPs from other organisms. Its deduced protein product has a subunit M(r) of 41,414, and shows substantial sequence homology to the bacterial amidohydrolase family. The product of the S. cerevisiae...
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