Article
Conditions for single strand conformation polymorphism (SSCP)analysis with broad applicability: a study on the effects of acrylamide, buffer and glycerol concentrations in SSCP analysis of exons of the p53 gene).
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Feb 1996
Teschauer W, Mussack T, Braun A, Waldner H, Fink E
Abstract excerpt
We examined the influence of electrophoretic conditions on the detectability of small sequence alterations in DNA fragments by single strand conformation polymorphism (SSCP) analysis. Three acrylamide concentrations, 7.5, 14 and 20%, were selected, and all three gel types were prepared with four...
Topics
- Acrylamides
- Buffers
- DNA
- DNA, Single-Stranded
- Electrophoresis
- Exons
- Genes, p53
- Glycerol
- Humans
- Kinetics
- Mutation
- Polymorphism, Single-Stranded Conformational
- Temperature