Article
Characterisation of genomic RNA of Coxsackievirus B3 in murine myocarditis: reliability of direct sequencing of reverse transcription-nested polymerase chain reaction products.
Journal of virological methods - 1 Dec 1997
Zhang H, Soteriou B, Knowlson S, Theodoridou A, Archard L C
Abstract excerpt
SWR mice develop viral myocarditis histologically similar to the human disease following inoculation with a cardiovirulent Coxsackievirus B3 (CVB3), reactivated from a sequenced cDNA clone of Nancy strain. A sequence of 215 nucleotides, or 628 nucleotides in representative cases, of the 5'non-tra...
Topics
- Animals
- Coxsackievirus Infections
- Disease Models, Animal
- Enterovirus B, Human
- Gene Amplification
- Genome, Viral
- Male
- Mice
- Mice, Inbred Strains
- Mutation
- Myocarditis
- Polymerase Chain Reaction
- RNA, Viral
- Reproducibility of Results
- Sequence Analysis, RNA
- Sequence Homology, Nucleic Acid
- Transcription, Genetic