Article
In vitro transcription of the Leptomonas seymouri SL RNA and U2 snRNA genes using homologous cell extracts.
Molecular and biochemical parasitology - 1 Dec 1997
Huie J L, He P, Bellofatto V
Abstract excerpt
A cell-free transcription system for the spliced leader (SL) RNA gene of the trypanosomatid Leptomonas seymouri has been developed. Accurately initiated transcription was achieved using cell extracts and a template in which the transcribed region of the SL RNA was replaced with a guanosine-less s...
Topics
- Animals
- Base Sequence
- Cell Extracts
- Cell-Free System
- DNA, Protozoan
- Genes, Protozoan
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA Splicing
- RNA, Messenger
- RNA, Protozoan