Article
In vitro transcription of mutated Leishmania tarentolae spliced leader RNA genes approximates in vivo patterns.
Molecular and biochemical parasitology - 1 Dec 2000
Yu M C, Roberts T G, Sturm N R, Campbell D A
Abstract excerpt
To elucidate the process of transcription in the kinetoplastids and to aid in the purification of transcription factors, we have developed a transcriptionally-competent nuclear extract from Leishmania tarentolae for the study of the spliced leader (SL) RNA gene. The extract was competent to transcribe a tagged SL RNA gene. The in vitro SL RNA transcripts initiated accurately and their synthesis was dependent on...
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