Article
Transcription of a nematode trans-spliced leader RNA requires internal elements for both initiation and 3' end-formation.
The EMBO journal - 1 Jun 1990
Hannon G J, Maroney P A, Ayers D G, Shambaugh J D, Nilsen T W
Abstract excerpt
We have used block substitution mutagenesis and in vitro transcription to define sequence elements important for efficient initiation and 3' end-formation of the trans-spliced leader RNA (SL RNA) of the parasitic nematode Ascaris lumbricoides. These experiments indicate that the SL RNA has an unusual promoter structure containing elements which include the 22 nt trans-spliced leader exon itself. Efficient...
Topics
- Animals
- Ascaris
- Base Sequence
- Deoxyribonuclease I
- In Vitro Techniques
- Molecular Sequence Data
- Mutation
- RNA Splicing
- RNA, Messenger
- Restriction Mapping
- Transcription, Genetic