Article
Accurate transcription of the Trypanosoma brucei U2 small nuclear RNA gene in a homologous extract.
The Journal of biological chemistry - 21 Jul 1995
Günzl A, Tschudi C, Nakaar V, Ullu E
Abstract excerpt
In vitro transcription systems are a classic means to dissect mechanisms of gene expression at the molecular level. To begin an analysis of the biochemistry of gene expression in trypanosomes, we established an in vitro transcription system from cultured insect forms of Trypanosoma brucei. As a model we used the U2 snRNA gene which in vivo is transcribed by an RNA polymerase with characteristics of animal RNA...
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