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Article

Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.

The EMBO journal - 1 Dec 1991

Maroney P A, Hannon G J, Shambaugh J D, Nilsen T W

Abstract excerpt

The spliced leader RNAs of both trypanosomes and nematodes can form similar secondary structures where the trans-splice donor site is involved in intramolecular base pairing with the spliced leader sequence. It has been proposed that this base pairing could serve to activate autonomously the SL RNA splice donor site. Here, we have examined exon requirements for trans-splicing in a nematode cell free system....

Topics

Animals
Base Composition
Base Sequence
Exons
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Nematoda
Nucleic Acid Conformation
Protein Sorting Signals
RNA Splicing

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Identifiers

PMID: 1935906
DOI: 10.1002/j.1460-2075.1991.tb04956.x
PMCID: PMC453124

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1935906-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.PMID 1935906Read ↗

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