Article
A generic sequencing based typing approach for the identification of HLA-A diversity.
Human immunology - 1 Oct 1997
Scheltinga S A, Johnston-Dow L A, White C B, van der Zwan A W, Bakema J E, Rozemuller E H, van den Tweel J G, Kronick M N, Tilanus M G
Abstract excerpt
Sequencing Based Typing (SBT) is a generic approach for the identification of HLA-A polymorphism. This approach includes the high resolution typing of the HLA-A broad reacting groups, HLA-A subtypes and will identify new alleles directly. The SBT approach described here uses a locus specific amplification of DNA from exon 1 to exon 5. The resulting 2,022 bp PCR product serves as a template for the subsequent...
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