Article
Genomic targeting of a bicistronic DNA fragment by Cre-mediated site-specific recombination.
Gene - 12 Dec 1997
Kolb A F, Siddell S G
Abstract excerpt
The Cre-recombinase of bacteriophage P1 catalyses site-specific recombination between DNA fragments containing loxP sites. Targeting of predefined genomic loci can be achieved by Cre-mediated linkage of a promoterless resistance marker gene to a floxed promoter pre-existing in the genome. In orde...
Topics
- Animals
- Animals, Genetically Modified
- Bacteriophage P1
- Blotting, Southern
- Cats
- Cell Line
- Cloning, Molecular
- DNA
- Gene Targeting
- Genes, Reporter
- Integrases
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Recombination, Genetic
- Transfection
- Viral Proteins