Article
Use of protein A gene fusions for the analysis of structure-function relationship of the transactivator protein C of bacteriophage Mu.
Protein engineering - 1 Aug 1997
De A, Paul B D, Ramesh V, Nagaraja V
Abstract excerpt
A sensitive dimerization assay for DNA binding proteins has been developed using gene fusion technology. For this purpose, we have engineered a gene fusion using protein A gene of Staphylococcus aureus and C gene, the late gene transactivator of bacteriophage Mu. The C gene was fused to the 3' end of the gene for protein A to generate an A-C fusion. The overexpressed fusion protein was purified in a single step...
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