Article
Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites.
Gene - 7 Feb 1997
Zeng X, Hu J C
Abstract excerpt
Chimeric proteins comprising the N-terminal DNA binding domain of lambda repressor fused to a fragment of a foreign protein have been used to detect oligomerization of the latter. Fusions containing dimeric and tetrameric leucine zipper domains can be distinguished based on their in vivo represso...
Topics
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- Chloramphenicol O-Acetyltransferase
- Crystallography, X-Ray
- DNA
- DNA-Binding Proteins
- Escherichia coli
- Leucine Zippers
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Recombinant Fusion Proteins
- Repressor Proteins
- Viral Proteins
- Viral Regulatory and Accessory Proteins
- beta-Galactosidase