Article
The isolated N-terminal DNA binding domain of the c repressor of bacteriophage 16-3 is functional in DNA binding in vivo and in vitro.
Molecular & general genetics : MGG - 1 May 1991
Dallmann G, Marincs F, Papp P, Gaszner M, Orosz L
Abstract excerpt
The 197 amino acid c repressor of the temperate Rhizobium meliloti phage 16-3 still regulates the OR operator of the phage after removal of its carboxyl terminal region. When cloned in the low-copy-number plasmid pGA46, a severely truncated variant (R1-77), which retains only the first 77 amino a...
Topics
- Alleles
- Amino Acid Sequence
- Bacteriophages
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Escherichia coli
- Genes, Regulator
- Genes, Viral
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Plasmids
- Promoter Regions, Genetic
- Repressor Proteins
- Rhizobium
- Terminator Regions, Genetic
- Transcription, Genetic