Article
A dual-affinity gene fusion system to express small recombinant proteins in a soluble form: expression and characterization of protein A deletion mutants.
Protein engineering - 1 May 1990
Jansson B, Palmcrantz C, Uhlén M, Nilsson B
Abstract excerpt
A novel gene fusion system to express and purify small recombinant proteins in Escherichia coli has been constructed. The concept allows for affinity purification of soluble gene products by sequential albumin- and Zn2(+)-affinity chromatography. The dual-affinity system is well suited for expres...
Topics
- Base Sequence
- Binding Sites
- Chromosome Deletion
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Immunoglobulin G
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Recombinant Proteins
- Serum Albumin
- Staphylococcal Protein A
- Zinc