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Article

The role of the 70 kDa subunit of human DNA polymerase alpha in DNA replication.

The EMBO journal - 1 Dec 1993

Collins K L, Russo A A, Tseng B Y, Kelly T J

Abstract excerpt

DNA polymerase alpha is the only enzyme in eukaryotic cells capable of starting DNA chains de novo and is required for the initiation of SV40 DNA replication in vitro. We have cloned the 70 kDa subunit of human DNA polymerase alpha (hereafter referred to as the B subunit) and expressed it as a fusion protein in bacteria. The purified fusion protein forms a stable complex with SV40 T antigen, both in solution and...

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Identifiers

PMID: 8223465
DOI: 10.1002/j.1460-2075.1993.tb06144.x
PMCID: PMC413886

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