Article
Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability.
Gene - 30 Nov 1990
Rojo F, Salas M
Abstract excerpt
The expression of Bacillus subtilis phage phi 29 late genes from the A3 promoter requires the viral protein p4. This protein is a transcriptional activator which binds to a region of the A3 promoter located between nucleotides -56 to -102, relative to the transcription start point. Mutants at the N terminus of protein p4 have been constructed and their function investigated. The binding of these deletion mutants...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacteriophages
- Chromosome Deletion
- DNA Fingerprinting
- DNA, Viral
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Genes, Viral
- Molecular Sequence Data
