Article
A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli.
Molecular & general genetics : MGG - 1 Jan 1998
Dmitrova M, Younès-Cauet G, Oertel-Buchheit P, Porte D, Schnarr M, Granger-Schnarr M
Abstract excerpt
Interactions between proteins affect a wide variety of biological processes, such as signal transduction and control of gene expression. In order to facilitate the study of protein-protein interactions we have developed a new method for specifically detecting the heterodimerization of two heterologous proteins in the bacterium Escherichia coli. The assay is based on the simultaneous use of protein fusions with an...
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