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Increasing detection of polymerase chain reaction (PCR) by isolation of PCR products (IPCRp).

Croatian medical journal - 1 Aug 2005

Dimsoski Pero, Woo Sam L

Abstract excerpt

AIM: To develop a method for enhanced polymerase chain reaction (PCR) product detection. METHODS: During the PCR, the double-stranded product is generated with fluorescent dye on one strand, and biotin on the other strand. The product is captured on the streptavidin-coated plates with high efficiency (IPCRp). Washing of the all unamplified compounds, including dye-labeled unincorporated primers, follows the PCR....

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PMID: 16100766

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