Article
Rapid electrochemiluminescence assays of polymerase chain reaction products.
Clinical chemistry - 1 Sept 1991
Kenten J H, Casadei J, Link J, Lupold S, Willey J, Powell M, Rees A, Massey R
Abstract excerpt
We demonstrate the first use of an electrochemiluminescent (ECL) label, [4-(N-succimidyloxycarbonylpropyl)-4'-methyl-2,2'- bipyridine]ruthenium(II) dihexafluorophosphate (Origen label; IGEN Inc.), in DNA probe assays. This label allows rapid (less than 25 min) quantification and detection of poly...
Topics
- 2,2'-Dipyridyl
- Base Sequence
- Coordination Complexes
- DNA Probes
- Electrochemistry
- Genes, ras
- Humans
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotides
- Papillomaviridae
- Polymerase Chain Reaction
- Sensitivity and Specificity
