Article
Quantitative analysis in molecular diagnostics.
Human pathology - 1 Jun 1994
Crotty P L, Staggs R A, Porter P T, Killeen A A, McGlennen R C
Abstract excerpt
Quantitative analysis of DNA products derived from polymerase chain reaction (PCR)-based assays depends on the careful optimization of each of the reaction parameters to achieve highly efficient amplification of target sequences. In practice, however, measurement of the accumulated PCR product is reliable only when analyses are performed at points in the exponential phase of the PCR amplification curve and before...
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