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Article

Pyrophosphorolysis by Type II DNA polymerases: implications for pyrophosphorolysis-activated polymerization.

Analytical biochemistry - 1 Jan 2004

Liu Qiang, Sommer Steve S

Abstract excerpt

We find that Type II DNA polymerases can catalyze pyrophosphorolysis, the reverse reaction of DNA polymerization. This property is applied utilizing pyrophosphorolysis-activated polymerization (PAP), a method of nucleic acid amplification using serial coupling of pyrophosphorolysis and polymerization. PAP can be used for ultrarare allele detection (detection of minimal residual disease and cancer risk assessment...

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Identifiers

PMID: 14654041
DOI: 10.1016/j.ab.2003.08.037

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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