Article
Solution structures of staphylococcal nuclease from multidimensional, multinuclear NMR: nuclease-H124L and its ternary complex with Ca2+ and thymidine-3',5'-bisphosphate.
Journal of biomolecular NMR - 1 Sept 1997
Wang J, Truckses D M, Abildgaard F, Dzakula Z, Zolnai Z, Markley J L
Abstract excerpt
The solution structures of staphylococcal nuclease (nuclease) H124L and its ternary complex, (nuclease-H124L).pdTp.Ca2+, were determined by ab initio dynamic simulated annealing using 1925 NOE, 119 phi, 20 chi 1 and 112 hydrogen bond constraints for the free protein, and 2003 NOE, 118 phi, 20 chi...
Topics
- Calcium
- Computer Simulation
- Crystallography, X-Ray
- Genetic Variation
- Hydrogen Bonding
- Micrococcal Nuclease
- Models, Molecular
- Molecular Conformation
- Nuclear Magnetic Resonance, Biomolecular
- Protein Structure, Secondary