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Article

Magnetic resonance studies of the binding of oligonucleotide substrates to mutants of staphylococcal nuclease.

Proteins - 1 Jan 1994

Chuang W J, Gittis A G, Mildvan A S

Abstract excerpt

By a combination of NMR docking and model building, the substrate binding site on staphylococcal nuclease was found to accommodate a trinucleotide and to consist of three subsites, each interacting with a single nucleotidyl unit of DNA. Binding of the essential Ca2+ activator and substrate cleavage occur between subsites 1 and 2. Hence, catalytically productive binding would span subsites 1 and 2 while...

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Identifiers

PMID: 8146123
DOI: 10.1002/prot.340180109

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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