Magnetic resonance studies of the binding of oligonucleotide substrates to mutants of staphylococcal nuclease.

doi:10.1002/prot.340180109

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Article

Magnetic resonance studies of the binding of oligonucleotide substrates to mutants of staphylococcal nuclease.

Proteins - 1 Jan 1994

Chuang W J, Gittis A G, Mildvan A S

Abstract excerpt

By a combination of NMR docking and model building, the substrate binding site on staphylococcal nuclease was found to accommodate a trinucleotide and to consist of three subsites, each interacting with a single nucleotidyl unit of DNA. Binding of the essential Ca2+ activator and substrate cleava...

Topics

Calcium
Dinucleoside Phosphates
Magnetic Resonance Spectroscopy
Manganese
Micrococcal Nuclease
Models, Chemical
Mutation
Oligodeoxyribonucleotides
Substrate Specificity

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